Doctor Patel in the lab

Immunohistochemistry

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Our division has one of the most sophisticated specialty laboratories in the region. The methods employed involve the use of antibodies or small fragments of DNA as probes to analyze cell samples or tissue specimens and address numerous common problems in diagnostic pathology.  

ProPath offers particular expertise in these areas, including the determination of the primary site in metastatic tumors of unknown origin, classification of tumors that appear 'undifferentiated' by standard light microscopy, precise classification of leukemias and lymphomas in routine specimens, determination of estrogen receptors, progesterone receptors, HER-2 overexpression status in breast cancer patients and identification of micrometastatic disease in sentinel lymph node biopsies taken from cancer patients. 

Our laboratory is also highly skilled in cell transfer and tissue transfer techniques, which allow the performance of batteries of immunostains on previously-stained slide material, when no paraffin block is available. In situ hybridization analysis is available for HPV subtyping and EBV (EBER), and fluorescent in situ hybridization (FISH) is also available for analysis of HER-2 gene amplification status in breast cancer patients. 

Thorough measures are taken to ensure and document quality control with every test performed. Multi-tumor sandwich block control sections are placed on the same slide as the diagnostic tissue and run with every specimen. These preparations contain up to 80 different tumors of all types in a compact grid arrangement that can be mounted on one-half of the stained slide, allowing the interpreting pathologist to easily and quickly ascertain that the test is sensitive, specific and performing as expected.

 

ProPath's Immunohistochemistry Laboratory is a CAP-accredited laboratory directed by Rodney T. Miller, M.D., an experienced diagnostic pathologist and immunohistochemist with a broad background in methodology and applications of Immunohistochemistry. Dr. Miller has published a number of articles on diagnostic and technical Immunohistochemistry, and has lectured on the subject at numerous national, international, and regional pathology and histotechnology meetings.